Threshold effect of caffeic acid on peroxidase-catalysed oxidation of indole-3-acetic acid
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چکیده
منابع مشابه
Threshold Effect during Inhibition by Caffeic Acid of Peroxidase-catalysed Oxidation of Indol Yl-3-acetic Acid*
Chemiluminescence has been used 10 study aerobic peroxidase-catalysed oxidation of indolyI-3-acetic acid (IAA). A threshold effect was found on inhibition of this reaction by its natural inhibitor caffeic acid. At an inhibitor concentration below threshold, only a temporary fall in the intensity of chemiluminescence and the reaction rale was established; at an above-threshold concentration, che...
متن کاملPeroxidase-catalyzed Oxidation of Indole-3-acetic Acid.
The aerobic oxidation of indole-3-acetic acid catalyzed by horseradish peroxidase produces 1268 nm emission characteristic of singlet oxygen. Lactoperoxidase also oxidizes indole-3-acetic acid to produce singlet oxygen, but in contrast to horseradish peroxidase, this enzyme system requires hydrogen peroxide. In both of these systems, the intensity of the 1268 nm emission is small due to quenchi...
متن کاملStudies on the oxidation of indole-3-acetic Acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic Acid oxidation products.
The method described here is based on a brief report by Harley-Mason and Archer. It involves the use of p-dimethylaminocinnamaldehyde (DMACA), a vinylogue of Ehrlich's reagent, as a color reagent for indoles. Colorimetric analyses of indoleacetic acid (IAA) oxidation reaction mixtures were made with the DMACA reagent as a solution rather than a spray. DMACA reagent will yield a wine-red color w...
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The stability of 21 amino acid conjugates of indole-3-acetic acid (IAA) toward horseradish peroxidase (HRP) was studied. The IAA conjugates of Arg, Ile, Leu, Tyr, and Val were oxidized readily by peroxidase. Those of Ala, beta-Ala, Asp, Cys, Gln, Glu, Gly, and Lys were not degraded and their recovery was above 92% after 1 hour incubation with HRP. A correlation between the stability of IAA conj...
متن کاملHydrogen Peroxide-mediated Oxidation of Indole-3-acetic Acid by Tomato Peroxidase and Molecular Oxygen.
The oxidation of indole-3-acetic acid by anionic tomato peroxidase was found to be negligible unless reaction mixtures were supplemented with H(2)O(2). The addition of H(2)O(2) to reaction mixtures initiated a period of rapid indole-3-acetic acid oxidation and O(2) uptake; this phase ended and O(2) uptake fell to a low level when the H(2)O(2) was exhausted. The stoichiometry of the reaction, wh...
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ژورنال
عنوان ژورنال: Phytochemistry
سال: 1993
ISSN: 0031-9422
DOI: 10.1016/0031-9422(93)85387-7